Contact an Account ManagerDetermination of arachidonic acid by on-line solid-phase extraction HPLC with UV detection for screening of cytosolic phospholipase A2a inhibitors
Summary
Type: HPLC
Application #: 126050
Detection:
Conditions: On-line SPE-HPLC/UV system with column switching
Injection:
- valve position 1: 400 µL sample, loaded on trap column with eluent A and flow rate A
- valve position 2: after 2 min back-flushing off the trap column onto analytical column with eluent B and flow rate B
- after about 30 injections the trap column was washed with 200 µL isopropanol
Eluent A: acetonitrile – water (20:80, v/v)
Eluent B: acetonitrile – water – 85% phosphoric acid (77:23:0.1, v/v/v)
Flow rate A: 0.5 mL/min
Flow rate B: 0.4 mL/min
Autosampler temperature: 10 °C
Column temperature: 20 °C
Authors: Hanekamp, W.; Lehr, M.
Source: J. Chromatogr. B 900 (2012), 79–84
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